Work

Protocol for brain and ventricle volume comparison after clearing and light-sheet microscopy in mice

Year: 2026

Type: article

Abstract: Analysis of brain and ventricular volumes provides insights into the physiological and pathological progression of brain development and aging, but accessible and cost-effective approaches are lacking. Here, we present a workflow to quantify relative brain and ventricular volumes in unsectioned cleared mouse brains following fluorescent detection of brain tissue and ventricle lining. We describe steps for brain harvesting, clearing, imaging by light-sheet microscopy, image resampling, and ventricle annotation, and comparing brains from adult, neonate, germ-free, and hydrocephalic mouse models.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104877

Authors: Ana Blas-Medina, Minh Son Phan, Samir Ali-Moussa, Nathalie Spassky, Julien Fernandes, Jean-Yves Tinevez, Aleksandra Deczkowska, Laetitia Travier

Institutions: Inserm, Institut Pasteur, Université Paris Cité, Sorbonne Paris Cité, Centre National de la Recherche Scientifique, Université Paris Sciences et Lettres, Institut de Biologie de l'École Normale Supérieure

Language: English

Topic: Cerebrospinal fluid and hydrocephalus

Subfield: Clinical Neurology

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funders: Alzheimer's Association, Biogen, “la Caixa” Foundation, Agence Nationale de la Recherche, Fondation pour la Recherche Médicale, Institut Pasteur, Conseil Régional, Île-de-France, Fédération pour la Recherche sur le Cerveau, European Research Council

Awards: Circadian Regulation of the Choroid Plexus in Brain Aging., G2772962269, Differential use of a multiciliation gene module in Choroid Plexus and Cajal-Retzius neurons, G598773271, Roles of mTOR pathway in glial development and in TORopathies, Establishment of the immune-brain communication channel at the choroid plexus and its role in brain development, France-BioImaging (Biological Imaging Next-Generation Instrument), G9000850769, G9000851115, G9000851628

OpenAlex ID: W7215098813

FWCI:

Cites: 17

Cited by:

Related to: 10