Work

Fast dynamic MPI cytometry

Year: 2023

Type: article

Abstract: We developed in vivo fast dynamic MPI “cytometry” to quantify and localize the accumulation of SPIO-labeled stem cells in different organs after intra-arterial injection on a time scale of minutes. Bone marrow-derived mesenchymal stem cells (MSCs) and superoxide dismutase 1 gene-corrected neural precursor cells (NPCs) were labeled with Resovist and injected into Rag2 mice using four separate injections. Whole body standard 2D/3D MPI scans were obtained, quantified and co-registered with CT. Using cell calibration fiducials, cells could be clearly visualized and quantified by MPI in vivo in the brain, liver, and lung. The cytometric ratio of the number of cells in the liver/lung vs. the brain was 1.5 for MSCs and 15.6 for NPCs, respectively, at 24 min post-injection. Fast dynamic MPI cytometry may find applications for optimizing the dose, volume, speed and route of administration when performing interventional cell therapy procedures in real-time.

Source: International journal on magnetic particle imaging

Authors: Shakeri-Zadeh, Ali, Kuddannaya, Shreyas, Bibic, Adnan, Walczak, Piotr, Bulte, Jeff

Institution: Johns Hopkins University

Language: English

Topic: Characterization and Applications of Magnetic Nanoparticles

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W7216734290

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