Work

Explants sterilization through metal nanoparticles for in vitro mass propagation of Eugenia involucrata

Year: 2019

Type: article

Abstract: An efficient method for the sterilization of explants is essential for the establishment of plant micropropagation. In this study we aimed to evaluate the efficacy of treatments using silver and gold nanoparticles as disinfectant agents of explants of Eugenia involucrata, in comparison to results from three other sterilization procedures. The sterilization procedure based on serial immersions in ethanol, sodium hypochlorite, and silver nanoparticles was quite effective, providing a very low proportion of explants contamination in nodal segments and apical buds of E. involucrata. The same protocol using gold instead of silver nanoparticles did not control fungi contamination of the explants. The immersion of explants in an in-house prepared solution of silver nanoparticles revealed to be an easy, cheap and efficient method for sterilization of nodal segments and shoot apices of E. involucrata, while the gold nanoparticles treatment was ineffective for controlling fungal contamination in this study.

Source: Plant Cell Culture & Micropropagation

Authors: Illamas Gallon, Fernanda, Roberto Diniz da Silva, Paulo, Czarnescki da Silva, Denise, Bisogno de Castro, Ferdinando, Marcos Stefenon, Valdir

Institutions: Universidade Federal de Lavras, Universidade Federal do Pampa

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: bronze

Funders: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Universidade Federal do Pampa

Awards: G3098311640, G7038554653, G7708484663

OpenAlex ID: W7217722562

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