Work

Gas chromatography-mass spectrometry profiling, bioactive compounds and cytotoxicity of edible-cricket extracts as inhibitors for α-glucosidase, α-amylase, tyrosinase, and acetylcholinesterase

Year: 2023

Type: article

Abstract: Importance of the work: In Thailand, consumption is increasing of edible crickets as a future prospective food, due to their various nutrient components and high level of proteins.Objectives: The crude extracts of several edible cricket insect species were investigated for their levels of enzyme inhibition activity regarding diabetes, Alzheimer's disease and melanogenesis.Materials & Methods: Several bioactive compounds, including alkaloids, saponins, steroids, triterpenoids, tannins, phenolics, and flavonoids, were investigated in the cricket extracts using the solvent extraction method and subsequently analyzed using gas chromatography-mass spectrometry (GC-MS) profiling and their inhibitory levels regarding α-glucosidase, α-amylase, tyrosinase and acetylcholinesterase. Results: The total phenolic and flavonoids in the methanol extracts had greater inhibition levels than the other extracts.α-Glucosidase and acetylcholinesterase activities were inhibited by the cricket extracts in dichloromethane, similar to standard drugs.The crude extracts of the short-tailed cricket had the same anti-tyrosinase ability as kojic acid.These extracts showed no cytotoxicity in mouse subcutaneous connective tissue and human keratinocyte immortal cells.The GC-MS analysis confirmed that the inhibitory activity levels of the unsaturated and saturated fatty acids were the major components in the cricket extracts.Main finding: Cricket extracts from the domestic house cricket, field cricket and short-tailed cricket, displayed diverse potential as enzyme inhibitors for α-glucosidase, α-amylase, tyrosinase and acetylcholinesterase, supporting the future use of crickets as functional food ingredients.

Source: Agriculture and Natural Resources

DOI: 10.34044/j.anres.2023.57.6.02

Authors: Jintana Masoongnoen, Sompong Sansenya, Kanokorn Wechakorn

Institution: Rajamangala University of Technology

Language: English

Topic: Insect Utilization and Effects

Subfield: Insect Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: Rajamangala University of Technology Thanyaburi, Thailand Science Research and Innovation