Analysis of gene expression patterns in various topographic sites of gastritis patients infected with Helicobacter pylori
Year: 2026
Type: article
Study design: Observational Study
Abstract: Background and Objectives: Helicobacter pylori infection primarily contributes to gastric carcinogenesis. This study ex- amined the expression of cyclin E1, β-catenin, PPAR-δ, and miR-9 in different gastric regions of gastritis patients with and without H. pylori infection. Materials and Methods: Gastric biopsies were obtained from 108 participants divided into three groups, including healthy (n = 15), gastritis (n = 53), and H. pylori-positive gastritis (n = 40). Bacterial culture and molecular characterization were performed to confirm H. pylori presence. Gene expression was assessed using SYBR Green-based RT-qPCR. Statistical analyses revealed correlations between the expression of cyclin E1, β-catenin, PPAR-δ, miR-9, and the presence of H. pylori in patients with gastritis compared to healthy controls. Results: The cagA-positive strains were significantly associated with the expression of cyclin E1, β-catenin, PPAR-δ (P < 0.001), and miR-9 (P < 0.01). Cyclin E1 and β-catenin expression were upregulated in gastritis and H. pylori-positive gastritis patients. The PPAR-δ expression was highest in H. pylori-positive gastritis patients, specifically in the antrum tissues. miR-9 showed significant downregulation in H. pylori-positive gastritis samples. Conclusion: cagA-positive H. pylori infection was linked to distinct gene expression changes that varied by gastric region. Further studies are needed to explore the mechanisms involved
Source: Iranian Journal of Microbiology
Language: English
Subfield: Gastroenterology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7219759584