A simple, rapid and improved colorimetric assay for nontransferrin thalassemia patients bound iron estimation inthalassemia patients
Year: 2018
Type: article
Abstract: Iron in the plasma that is not attached to the protein transferrin, which normally carries it, is referred to as"non transferrin bound iron." It has been hypothesized that the redox activity of such iron compounds, asshown by Haiber weiss and Fenton chemistry, makes them poisonous. Non transferrin bound iron has beenthe subject of much research in patients receiving blood transfusions for the treatment of iron overloaddisorders such as hemochromatosis and thalassemia. Several academics have proposed studying NTBI toevaluate the usefulness of chelators in treating iron excess. Different researchers have proposed differentwaysfor measuring the amount of iron that is not bound to such as chromatography, atomic absorption spectroscopy, etc. The considerable complexity of the process, theneed for expensive specialized equipment and accessories, and the questionable dependability mean thatnone of them is still universally recognized or regarded gold standard. In order to make thebathophenanthroline based colorimetric approach for non transferrin bound iron measurement accessible tosmaller labs with less resources without sacrificing accuracy, we have experimented with a number ofalternative variants. NTBI values were calculated for both -thalassemia patients and healthy controls usingthe trial methodology that yielded the most stable findings. We discovered that the background noise wasmuch reduced and the most consistent findings were produced by high-speed centrifugation with interstitialsample pre-incubation. There was a statistically significant difference between the means of the thalassemiapatient group and the control group for the quantity of non-transferrin bound iron.Hemochromatosis, betathalassemia, and bathophenanthroline are all diseases associated with an excess of iron that isn't bound totransferrin.
Author: L.Laxi kumari
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7220282221