Year: 2026
Type: preprint
Abstract: ABSTRACT In the enzymatic toolbox of depollution, hydrolytic dehalogenases are of particular interest for the bioremediation of organohalogenated compounds. To better identify and characterize these enzymes, it is important to develop appropriate sensing methods. Fluorescent probes for dehalogenase detection have been developped in the past years, however, to this date none were available for application using the common FITC parameters ( i.e. excitation laser at 488 nm). To adress this limitation, we herein report a new fluorescent probe, “COU4-Cl” excitable in the violet (405 nm) and blue (488 nm) wavelengths adapted for the detection and characterization of haloalkane dehalogenases. Its applicability is discussed and evaluated in comparison to the previously described COU2-Cl probe only excitable in the violet (405 nm). This study shows the advantages and complementarity of the two probes, and further demonstrate their application for the discrimination of dehalogenase activity in whole cell assays. GRAPHICAL ABSTRACT
Institutions: Lyon 1 Université, Centre National de la Recherche Scientifique, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Laboratoire de Microbiologie, Adaptation et Pathogénie, Institut National des Sciences Appliquées de Lyon, École d'Ingénieurs en Chimie et Sciences du Numérique
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Award: G4853991302
OpenAlex ID: W7220461332