ผลของสารสกดจากเมลดกระทงลาย (Celastrus paniculatus Willd.) ตอการมชวตของเซลล ประสาทฮปโปแคมปสและซรบรล คอรเทกซในหนแรทเพศผ
Year: 2023
Type: article
Abstract: Background: Celastrus paniculatus (CP) seed extract has been reported to have neuroprotective and antioxidant activities. However, the direct effect of CP seed extract on promoting neuronal survival is unknown. Objective: The present study aimed to demonstrate the effects of CP seed extract on neuronal apoptosis and histological change in the brain of male Sprague Dawley rats. Materials and Methods: This study is a prospective, randomized, controlled study in 4-weekold rats. There were 12 male rats, 6 rats in the experimental group was injected with CP seed extract at the dose of 80 mg/kg body weight intraperitoneally daily for 2 weeks. Six control groups were injected with Dimethylsulfoxide (DMSO) 0.5% 0.2 mL volume daily for 2 weeks as well. Rats were rested for 5 days. The hippocampal structure was observed by hematoxylin and eosin staining technique. The expression of cerebral and hippocampal BCL-2 protein wasperformed by using immunohistochemistry technique. The expression ratio of BCL-2 to BAX in whole brain protein was studied by Western blot technique. Body weight, food intake, and water intake of control and experimental rats were recorded throughout 14 days of treatment. Results: Body weight, food intake, and water intake of control and experimental rats were comparable; indicating that CP seed extract has no effect on metabolic aspect of the rats. Hippocampal structure and neurons were also comparable in both groups; suggesting that CP seed extract has nontoxicity effect. However, the cerebral and hippocampal BCL-2 expression were significantly increase in treated group when compared with those of the control group. Whole brain BCL-2/BAX expression ratio was also significantly increased in treated group when compared with the control group. Since it has been suggested that the ratio of BCL-2 to BAX is an important determinant of neuronal survival, CP-regulated BCL-2/BAX expression may play a vital role in neuronal protection beyond their antioxidant activity. Conclusion: CP seed extract had no neurotoxic effect and enhanced cell survival through inhibition of apoptosis.
Source: Burapha Journal of Medicine
Institution: Burapha University
Language: Thai
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7220582309