Dissecting functional dynamics of fecal-derived in vitro stable microbial communities with metaproteomics
Year: 2026
Type: preprint
Abstract: Summary Fecal-derived in vitro microbial communities represent promising models for investigating gut microbiome function and facilitating translational applications. However, the factors governing community establishment and the underlying mechanisms remain incompletely understood. We employed large-scale quantitative metaproteomics to investigate the functional and community dynamics of human fecal microbiomes cultured in three media over 25 serial passages. Our results demonstrated that both culture medium and donor-specific microbiome characteristics significantly influence community establishment. Taxon-specific functional analyses revealed nutrient availability in the medium and the microbial stress responses as key determinants of in vitro community establishment. Notably, the metabolism of mucin glycans in RapidAIM medium contributed to the maintenance of Bacteroidaceae species that were poorly supported in other media, resulting in an in vitro community that most closely resembled the uncultured fecal inoculum. Overall, these findings provide mechanistic insights into in vitro gut microbial community establishment, supporting further optimization and application of fecal-derived in vitro microbiome models.
Language: English
Topic: Gut microbiota and health
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: Government of Canada, Health Canada
OpenAlex ID: W7220685504