Validation of the newly developed PlantECG fluorometer against three established chlorophyll fluorescence devices under progressive drought stress in pepper, tomato, and strawberry plants
Year: 2026
Type: article
Abstract: Comparing chlorophyll fluorescence data across different fluorometers remains challenging. We compared the new PlantECG fluorometer with PocketPEA, HandyPEA, and FluorPen in pepper (Capsicum annuum L.), tomato (Solanum lycopersicum L.), and strawberry (Fragaria ananassa Duch.) under progressive drought (0-96 h without irrigation). The maximum quantum yield of PSII photochemistry (FV/FM) measured by PlantECG correlated strongly with all reference devices (R2 = 0.72-0.98), although PlantECG slightly overestimated FV/FM under severe stress. The performance index (PIABS) showed greater inter-device divergence, with strong correlations in pepper and strawberry (R2 = 0.81-0.89) but weaker ones in tomato (R2 = 0.59-0.67). Absolute PIABS values differed substantially: PlantECG values were closest to PocketPEA, whereas HandyPEA recorded 2-5-fold lower values. Correlation quality depended on the species-specific rate of drought response. PlantECG reliably tracks drought-induced changes in photosynthetic performance, but species-specific calibration is required before comparing its absolute PIABS values across instruments.
Source: Photosynthetica
DOI: 10.32615/ps.2026.031
Authors: H.M. KALAJI, P. DĄBROWSKI, A. AURIGA
Institution: Warsaw University of Life Sciences
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $3,140
OpenAlex ID: W7220833743
