Contact of mobile cis -Golgi network with ER exit sites executes COPI-mediated receptor recycling
Year: 2026
Type: preprint
Abstract: COPI-mediated retrograde transport is essential for maintaining homeostasis between the endoplasmic reticulum (ER) and the Golgi in eukaryotic cells. However, its spatiotemporal dynamics remain unclear. In budding yeast, ER export occurs through a “hug-and-kiss” mechanism, wherein mobile cis -Golgi network (CGN) elements transiently approach ER exit sites (ERES) to capture cargo before departing to deliver it to the Golgi. Here, using high-resolution 3D and 4D live imaging, we show that the COPI-mediated recycling of a cargo receptor from the CGN back to the ER occurs during the hug-and-kiss event. Furthermore, this recycling is complete before the CGN detaches from the ERES, suggesting that COPI-mediated retrograde transport is spatiotemporally coupled to ER export during a single and transient CGN-ERES association. Consistent with this model, when COPI function is impaired, the receptor fails to return efficiently to the ER and instead remains in the CGN after separation from the ERES. Additionally, COPI impairment prolonged CGN-ERES association, suggesting that COPI function also facilitates CGN detachment from ERES. Thus, we propose that rapid COPI-mediated recycling drives CGN maturation directly at the ERES interface, enabling the CGN to detach and proceed along the secretory pathway.
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
Funders: Ministry of Education, Culture, Sports, Science and Technology, Ministerio de Educación, Cultura y Deporte, Ohsumi Frontier Science Foundation, Japan Society for the Promotion of Science, European Regional Development Fund, Agencia Estatal de Investigación, Core Research for Evolutional Science and Technology
Awards: Comprehensive analysis of the molecular mechanisms underlying prespore membrane morphogenesis, G3172253082, Deciphering the mechanisms of neuronal circuit formation and regeneration through high-speed super-resolution imaging and local manipulation of membrane traffic, G3393685150, G410571670, Construction of a unified model on membrane traffic around the Golgi, G4349142073, G451917667, G456322061, Elucidation of the molecular mechanism for accurately and efficiently retrograde cargo transport from the Golgi to the ER, G5958435931, G5967599077, G6457043867, G6705908148, G685327026, 古代説話における諸寺縁起の研究, G77066809, Full elucidation of sorting mechanisms in and around the Golgi apparatus by super-resolution live imaging
OpenAlex ID: W7220858355