Patch-seq analysis reveals transcriptomic correlates of neuronal firing rate adaptation across subtypes and species
Year: 2026
Type: article
Abstract: Can the transcriptomic profile of a neuron predict its physiological properties? Using a Patch-seq dataset of the primary visual cortex, we addressed this question by focusing on spike rate adaptation (SRA), a well-known phenomenon that depends on small conductance calcium (Ca)-dependent potassium (SK) channels. We first show that in parvalbumin-expressing (PV) and somatostatin-expressing (SST) interneurons, expression levels of genes encoding the ion channels underlying action potential generation are correlated with the half-width (HW) of spikes. Surprisingly, the SK encoding genes are not correlated with the degree of SRA (dAdap). Instead, genes that encode proteins upstream from the SK current, as well as the low-voltage-activated calcium channel, are correlated with dAdap. This general principle is supported by datasets from the mouse primary motor cortex and multiple macaque brain regions. Finally, we generate testable predictions by constructing a minimal computational model.
Source: iScience
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,240
Funders: National Science Foundation, Foundation for the National Institutes of Health, Simons Foundation, Canada First Research Excellence Fund, National Institutes of Health, Canadian Institutes of Health Research, Natural Sciences and Engineering Research Council of Canada, Office of Naval Research, National Institute of Mental Health, National Institute of Neurological Disorders and Stroke, Common Fund, Next Generation Network for Neuroscience
Awards: G1348234692, G1550933007, G196073109, Development and Function of 5HT3aR-Expressing Cortical GABAergic Interneurons, Distributed dynamics & cognition in a large-scale primate cortical circuit model, G4045788911, NEURONEX: The fabric of the primate neocortex and the origin of mental representations. From transcriptomics to single neurons and neuronal networks., G4609419830, Cholinergic Mechanisms of Associative Learning in Cortical Circuits, NEURONEX: The fabric of the primate neocortex and the origin of mental representations. From transcriptomics to single neurons and neuronal networks., Functional diversity of cholinergic streams modulating cognition, G8312533381, G8876996369, Inhibitory and Disinhibitory VIP Interneuron-Mediated Circuits in Neocortex, Development of genetic approaches to study the organization and function of neocortical layer 1, Development of Genetic Strategies to study a novel disinhibitory system in the cortex
OpenAlex ID: W7220940075