Work

KDM5A overexpression modulates key myogenic differentiation factors and sarcomere gene expression

Year: 2026

Type: article

Abstract: Myogenic differentiation is a complex and tightly regulated process involving multiple transcriptional regulators. KDM5A (lysine demethylase 5A) is a histone demethylase that regulates gene transcription and has been implicated in skeletal muscle differentiation. In Inclusion Body Myositis, we previously associated dysregulated myogenic differentiation with KDM5A overactivity. Immortalised human myoblasts overexpressing KDM5A showed an altered transcriptional landscape, with differential expression of key myogenic differentiation factors and significantly increased expression of sarcomeric genes. Although no morphological differences were observed, KDM5A-overexpressing myotubes showed a higher proportion of myogenin-positive nuclei during late-stage differentiation, suggesting that KDM5A modulates myogenic differentiation dynamics.

Source: PubMed Central

DOI: 10.17912/micropub.biology.002136

Authors: Geert de Vries, Alice Monticelli, Vicky De Winter, Danique Beijer, Willem De Ridder, Jonathan Baets

Language: English

Topic: Muscle Physiology and Disorders

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Association Belge contre les Maladies Neuro-Musculaires, Fonds Wetenschappelijk Onderzoek, Universiteit Antwerpen, Vlaamse regering

Awards: G1901141725, Neuromuscular disorders: from the omics-age towards novel therapies, G6970579791