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LC-ESI/MS/MS profile of purslane (Portulaca oleracea) nanoparticles for reduction foodborne pathogens and enhancing quality parameters of guava nectar model

Year: 2026

Type: article

Abstract: Purslane ( Portulaca oleracea ) is a naturally growing weed, containing a lot of polyphenols. This study aimed to evaluate purslane nanoparticles (PUR-NP) as antimicrobial and antioxidant in guava nectar storage at 4 ± 1 ℃ for 6 weeks. PUR-NP were incorporated into freshly made guava nectar at 100, 200, 300, and 400 µg mL − 1 , and compared with natamycin 40 µg mL − 1 and a control positive (pasteurized) as a reference, and without addition as the control sample. The LC-ESI/MS/MS profile, antimicrobial activity, and antioxidant ability of PUR-NP were performed, as well as the physicochemical properties, and quality parameters of guava nectar were done. Results showed that PUR-NP (55 nm) exhibited high antimicrobial activity at 400 µg mL − 1 opposite E. coli O157:H7 , S. aureus , penicillium expansum , and Candida glabrata . A noteworthy distinction was noted between PUR-NP treatments and natamycin. Fifteen major polyphenol compounds were identified in the PUR-NP extract and exhibited high antioxidant capacity compared to purslane fresh leaves (PFL) or purslane dried leaves (PDL). A noteworthy distinction in physicochemical attributes was noted between the guava nectar including PUR-NP 100–400 µg mL − 1 and control. However, no discernible variation were noted between guava nectar containing PUR-NP and natamycin. Results showed that PUR-NP at 400 µg mL − 1 were more effective as an antimicrobial and antioxidant that improves the quality attributes and shelf-life in guava nectar.

Source: Scientific Reports

DOI: 10.1038/s41598-026-74991-6