Year: 2026
Type: article
Abstract: Introduction Salmonella infection remains a pervasive diarrheal illness, particularly affecting populations in developing countries. The lack of an effective vaccine against non-typhoidal Salmonella is attributable to antigenic diversity among serotypes, incomplete understanding of protective immune mechanisms, and the difficulty in identifying conserved antigens capable of eliciting broad cross-protective immunity. Methods To identify conserved Salmonella antigens, we established a Salmonella -specific monoclonal antibody library using heat-killed bacteria. Candidate antigens recognized by monoclonal antibodies were characterized, and their functional relevance was evaluated through analyses of bacterial growth, respiratory activity, and protective effects in a murine Salmonella infection model. Results We identified L2-1H11, a monoclonal antibody that recognizes OppA, a conserved antigen present across multiple Salmonella serotypes. L2-1H11 was associated with suppression of bacterial growth and respiratory activity and attenuated enteritis pathology in Salmonella -infected mice. Conclusion These findings identify OppA as a conserved Salmonella antigen and suggest that it may represent a promising target for the development of subcutaneous and oral vaccines against Salmonella .
Source: Frontiers in Immunology
Institutions: National Institute of Biomedical Innovation, Health and Nutrition, Biomanufacturing Process Research Center, National Institute of Advanced Industrial Science and Technology, BIKEN Foundation, Research Institute for Microbial Diseases, The University of Osaka, The University of Osaka, Meiji University, Osaka Metropolitan University, Hiroshima University, Waseda University, Institute of Medical Science, The University of Tokyo, Kobe University, The University of Tokyo
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
Awards: G1264619005, The effects of environmental chemicals on ocular immune systems, G265389467, Identification of target molecules of functional food ingredients in the intestinal tract and analysis of their effects on the intestinal microflora, Assessement of relationship between gut dysbyosis and pathogenesis of pulmonary hypertension, Effects of lifestyle changes on gut microbiota and metabolites: N-of-1 crossover intervention study, G4087806862, G4252504591, G4271127948, カンピロバクター感染における自己免疫応答と神経障害の包括的理解と病態解明, The Effects of Environmental Factors on Immune Metabolism in Allergic and Inflammatory Diseases, G5296029281, G5302586464, G5771238525, ビフィズス菌特異的抗体ライブラリを活用した腸内細菌叢制御機構の解明, G5986500842, G6637831528, G7933571598, Understanding the mechanism of the cutaneous immune-diversity and its relationship with other organs, Understanding the mechanism of Campylobacter enteritis from the perspective of bacterial metabolism, G83060929, G8852034641
OpenAlex ID: W7221066784