A glycosaminoglycan degrading Polysaccharide Utilization Locus in the oral microbiota species Segatella oris
Year: 2026
Type: preprint
Abstract: The human gut microbiota has been explored for the last two decades and studies have uncovered its major role in the degradation of complex glycans from the diet as well as the host tissues. Strikingly, until very recently, no similar investigation has been carried out on the human oral cavity microbiota, which is increasingly recognized has an important parameter in gastrointestinal health. Prevotellaceae are an important and under-investigated family of bacteria in both the gut and the oral cavity microbiota. The oral cavity is particularly rich in glycosaminoglycans which could provide an abundant carbon source for the inhabiting microorganisms, and play a significant role in host-pathogen interactions. Hence, we analyzed Polysaccharide Utilization Loci in oral microbiota species and we identified a glycosaminoglycan-degrading Polysaccharide Utilization Locus in Segatella oris . We produced and characterized the four enzymes, confirmed their ability to degrade glycosaminoglycans, and we focused our efforts on the biochemical and structural characterization of the first reported Prevotellaceae endosulfatase.
Institutions: Centre National de la Recherche Scientifique, Centre de Recherches sur les Macromolécules Végétales, Université Grenoble Alpes, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Institut de Biologie Structurale, Université d'Orléans, Institut de Chimie Organique et Analytique
Language: English
Subfield: Periodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7221118632