Neooleiphilus portus gen. nov., sp. nov. and Paroleiphilus aggregans gen. nov., sp. nov., Two Novel Nitrate-Reducing Bacteria Isolated from Estuarine Sediments
Year: 2026
Type: article
Abstract: Two novel bacteria, designated strain G1-8T and J1-3T, were isolated from estuarine sediment samples collected from Jinjiang Estuary, Fujian Province, China, using the dilution-to-extinction method. Cells of strain G1-8T were Gram-stain-negative, aerobic, rod-shaped with a single polar flagellum, and had a surface layer. Cells of strain J1-3T were also Gram-stain-negative, aerobic, and short rod-shaped, yet the flagellum and the surface layer were not observed. Reduction of nitrate to nitrite under aerobic conditions was positive for both stains. Strains G1-8T and J1-3T shared the highest 16S rRNA gene sequence similarities with Oleiphilus messinensis ME102T of 94.45% and 93.77%, respectively. Phylogenetic analysis placed the two strains into two different monophyletic lineages, affiliated with the family Oleiphilaceae of the class Gammproteobacteria. The digital DNA-DNA hybridization, average nucleotide identity, and average amino acid identity between the two strains and O. messinensis ME102T were 21.2–22.6%, 68.6–68.8%, and 58.2–59.3%, respectively. The major fatty acids (>10%) of strain G1-8T were C16:0 (24.9%), C16:1 ω9c (14.9%), C18:1 ω9c (14.2%), and summed feature 3 (13.1%), and the major fatty acids (>10%) of strain J1-3T were summed feature 3 (39.6%) and C16:0 (37.0%). The isoprenoid quinone of both strains was Q-9. The polar lipids of strain G1-8T consisted of phosphatidylglycerol and two unidentified phospholipids, and the polar lipids of strain J1-3T consisted of phosphatidylethanolamine and two unidentified lipids. Based on the above polyphasic taxonomic study, strains G1-8T and J1-3T were considered to represent two novel species within two novel genera of the family Oleiphilaceae. Thus, two species names were proposed: Neooleiphilus portus gen. nov., sp. nov., with the type strain G1-8T (=MCCC 1K10098T=KCTC 18326T), and Paroleiphilus aggregans gen. nov., sp. nov., with the type strain J1-3T (=MCCC 1K10092T=KCTC 18325T).
Source: Microorganisms
Authors: Zhaobin Huang, Qiliang Lai
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,258
OpenAlex ID: W7221319263