Antibacterial effect of Bradyrhizobium spp., Pseudomonas spp., and Bacillus subtilis against pathogenic fungus Rhizoctonia solani on peanut plant
Year: 2022
Type: book
Abstract: Microorganisms such as fungi, bacteria, viruses, andnematodes are integral parts of agro-ecosystems. Some of them are harmfulto plant pathogens, whereas, others are neutral or beneficial in their effects onplant growth. The total agricultural losses of economic crops which amountto about 50–75% are caused by soil-borne pathogenic fungi of Rhizoctoniaspp., Fusarium spp., Verticillium spp., Sclerotinia spp., Pythium spp. AndPhytophthora spp. The losses are due to seed rot, root rot, and wilt diseasesin different crop fields and greenhousesAimes: the present study aimed to evaluate the antagonistic effect of thestudied bacteria i.e., Pseudomonas fluorescens, Pseudomonas chlororaphis,and Bacillus subtilis were tested against three tested isolates of R. solani Methods: The biocontrol tests were carried out using fungal and bacterialbioagents. The tested fungal bioagents were three isolates of Trichodermaviride. culture collection, three isolates for Rhizoctonia spp were Previouslyobtained from peanut plants with diseased roots. The bacterial antagonisticinclude one isolate of Bacillus subtilis and one isolate of Pseudomonasfluorescens.Results: Showed that Bacillus subtilis had the highest suppression effect(100%) with the three tested isolates of Rhizoctonia solani compared toPseudomonas fluorescens and P. chlororaphis.Pseudomonas fluorescens had the lowest bacterial in its suppressioneffect on the tested fungal growth R. solani 5 (16.7%), R. solani 3 (5.6%), andR. solani 12 (0.0%). Peanut seeds germinated with treated by Bradyrhizobiumisolates i.e., 208, 209, 210and Bradyrhizobium mixed, Bradyrhizobium +Trichoderma harzianum, Trichoderma alone, Bradyrhizobium +pseudomonas fluorescence results showed that followings: The isolateBradyrhizobium (208) gave the best germination percentage of peanut seeds(4.1667 cm length). The moderate germination of peanut seeds with the;Trichoderma harzianum, mixed Bradyrhizobium, and Bradyrhizobium + P.fluorescences had given (2.73, 2.40, and 2.33 cm of roots length). The lowestgermination of peanut seeds was obtained in the (control) treatment (1.43). Asignificant inhibitory effect on radial growth of the three tested R. solaniisolates due to Trichoderma viride isolates. Moreover, Trichoderma virideisolate 1 showed the highest inhibitory effect with three tested R. solani,(100%) with R. solani isolates 5 and 12, and R. solani isolates 3 (88.9%).
Source: Al-Qurtas
Institution: Al Zawiya University
Language: Arabic
Subfield: Plant Science
Domain: Life Sciences
SDGs: Zero hunger, Life on land
Open Access status: diamond
OpenAlex ID: W7221491526