Work

Deactivating siRNA activity with bioorthogonal inverse-electron-demand Diels–Alder chemistry

Year: 2026

Type: article

Abstract: Small interfering RNAs (siRNAs) were prepared with vinyl nucleobases and imperfect duplex reaction sites that enable selective inverse electron-demand Diels-Alder (IEDDA) reactions with tetrazines in living cells. These bioorthogonal reactions attenuate gene silencing of both reporter and endogenous genes, establishing a small-molecule strategy for deactivating siRNA activity.

Source: RSC Chemical Biology

DOI: 10.1039/d6cb00284f

Authors: Kaifeng Zhao, Kaleena Basran, Loïc Courville-Fortin, Nathan W. Luedtke

Institution: McGill University

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,962

Funders: Canada Foundation for Innovation, Canada First Research Excellence Fund, Natural Sciences and Engineering Research Council of Canada