Work

Acyl transfer activity inhibition and its application in moderate enzymatic degumming of crude soybean oil

Year: 2026

Type: article

Abstract: In this study, sodium tetraborate (Na 2 B 4 O 7 ) was used as an inhibitor to exploit the electron-withdrawing capability of boron atoms, it locks the activated lone pair electrons of hydroxyl oxygen and blocks the formation of the first intermediate, thereby inhibiting acyl migration. Under the optimized conditions of pH 6.0, temperature 60 °C, enzyme addition 0.02%, Na 2 B 4 O 7 addition 0.8%, and reaction time of 4 h, the phosphorus content in crude soybean oil was reduced to 12.1 mg/kg, while the FFA content increased to 1.05 g/100 g. Compared to conventional enzymatic degumming, the FFA content was reduced by 0.19 g/100 g, and the acyl migration rate was decreased by 17.0%. Liquid chromatography analysis confirmed that the addition of the inhibitor achieved moderate enzymatic degumming of crude soybean oil.

Source: Food Chemistry X

DOI: 10.1016/j.fochx.2026.104603

Authors: Ruichi Guo, Xiangyu Du, Shunian Luo, Liqi Wang, Fuming Yang, Dianyu Yu

Institutions: Harbin University of Commerce, Northeast Agricultural University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,330

Funder: Natural Science Foundation of Heilongjiang Province