Work

Bromo-desthiobiotin (Br-DTB) tag for unbiased profiling and identification of chemical modifications

Year: 2026

Type: article

Abstract: and l-azidohomoalanine (AHA) labelling in living cells. We further elucidated the covalent labelling site of piperlongumine (PL) on the E3 ligase KEAP1, demonstrating its utility for discovering new covalent labelling sites. Collectively, Br-DTB tags are suitable for chemoproteomic workflows, providing a robust platform for covalent labelling discovery and functional proteome profiling.

Source: RSC Chemical Biology

DOI: 10.1039/d6cb00192k

Authors: Bibhuti Bhusana Palai, Daniel H. Ramirez, Changfeng Deng, Francisca N. de Luna Vitorino, Chenfeng Zhao, Dong Zhu, Dongwen Lv, Benjamin A. Garcia, Zongtao Lin

Institutions: Washington University in St. Louis, Rutgers, The State University of New Jersey, Augusta University

Language: English

Topic: Click Chemistry and Applications

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,962

Funders: National Science Foundation, National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of Neurological Disorders and Stroke, Division of Chemistry

Awards: Hijacking Post-translational Arginylation for Targeted Protein Degradation, G301209757, G3137425078, A Mass Spectrometry Approach to the Genetic and Epigenetic Mechanisms Controlling Neuronal Identity, G757106693, Collaborative Research: MFB: Deciphering the Logic of PTM Crosstalk via Novel Chemical Technology: Histones and Beyond, Cancer Center Support Grant, Development and Application of Chemical Biology Approaches for Understanding Protein Arginylation, Development of Caspase Cleavage Targeting Chimeras (CACTACs) for Targeted Protein Cleavage.

OpenAlex ID: W7221597542

FWCI:

Cites: 23

Cited by:

Related to: 10