Work

Spatiotemporal Control of Organoid Biofabrication with Nanoengineered Bioinks

Year: 2026

Type: article

Abstract: Organoids are spatiotemporal developmental systems in which tissue architecture and function emerge through dynamically coordinated interactions among cells, extracellular matrix (ECM), biochemical signals, and mechanical cues. Because these regulatory inputs vary across both space and developmental time, organoid morphogenesis requires engineering strategies that precisely control their localization, magnitude, and temporal sequence. In this review, we examine nanoengineered bioinks and bioprinting strategies as complementary approaches for coordinating the spatial organization and temporal regulation of organoid development. We discuss how bioprinting establishes cell positioning, matrix architecture, biochemical gradients, and vascular-like structures within three-dimensional (3D) organoid models. We then examine how nanoengineered bioinks control factor release, matrix remodeling, mechanical evolution, and reciprocal cell-matrix interactions over time. Applications in brain, tumor, cardiac, skeletal muscle, and intestinal organoids illustrate how these design principles support tissue-specific patterning, multicellular interactions, and functional maturation. Finally, we highlight future directions for advancing organoid bioprinting toward predictive and developmentally responsive platforms, including adaptive bioinks, longitudinal monitoring, and data-guided optimization.

Source: BioChip Journal

DOI: 10.1007/s13206-026-00282-x

Authors: Seokgyu Han, Gwang Myeong Seo, Sungsu Park

Institutions: Brigham and Women's Hospital, Harvard University, Sungkyunkwan University

Language: English

Topic: 3D Printing in Biomedical Research

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,490

Funder: National Research Foundation of Korea