Physicochemical and biological profiles of a by-product of human donor plasma fractioning
Year: 2026
Type: article
Abstract: Aim: to perform an integrated physicochemical and biological analysis of fibrinogen-containing precipitate No. 1 (FР No. 1), a by-product of donor plasma fractionation, and to evaluate its potential as a raw material for biomedical applications. Material and methods. A multi-method approach was employed, including Fourier-transform infrared (FTIR) spectroscopy, UV spectrophotometry, scanning electron microscopy (SEM), SDSPAGE electrophoresis, quantitative determination of protein mass fraction, assessment of fibrinogen extraction yield in citrate buffer and phosphate-buffered saline (37 °C, 5 hours), as well as in vitro biological testing on human dermal fibroblast cultures. Results. FP No. 1 was found to preserve the structural integrity of fibrinogen with low levels of impurities. The lyophilizate formed porous fibrin matrices with pore sizes ranging from 100 to 180 μm. Cell viability upon contact with the material reached 96.5 % (p 0.05), confirming the absence of cytotoxicity. The protein mass fraction in the dry substance was 77 %. Under optimal extraction conditions, the fibrinogen yield reached 21.9 % (citrate buffer) and 21.5 % (phosphate-buffered saline). Conclusion. FP No. 1 represents a highly promising secondary protein raw material for the development of bioinks for 3D bioprinting, wound dressings, and tissue-engineered constructs. These findings strongly advocate for the rational reintegration of plasma fractionation byproducts into hightechnology biomedical applications.
DOI: 10.35693/sim706425
Institution: Samara State Medical University
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7221786325