Work

Lgr5 deficiency accelerates intervertebral disc degeneration via NF-κB activation and enhanced osteoclastogenesis

Year: 2026

Type: article

Abstract: Background: Intervertebral disc degeneration (IDD) is a common degenerative disease and the primary cause of low back pain. Leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) is an activator of the canonical Wnt signaling pathway. But its role in IDD progression and the underlying molecular mechanisms remain poorly understood. Methods: mice with Prx1-Cre transgenic mice. These samples were analyzed by RNAseq, micro-CT, histology and western blotting, etc. Results: MicroCT analysis of Lgr5 KO and wild-type (WT) mice caudal vertebrae showed that Lgr5 KO mice exhibited narrowed disc space, deteriorated trabecular bone microarchitecture, and decreased bone volume fraction (BV/TV). In a surgically induced IDD model, we showed that Lgr5 KO led to disrupted disc structure, nucleus pulposus (NP) atrophy, cellular loss, and decreased glycosaminoglycan content, thereby accelerating the progression of IDD. RNA-seq analysis identified 900 differentially expressed genes (457 upregulated, 443 downregulated) in Lgr5 KO discs, enriched in osteoclast differentiation, ECM-receptor interaction, and NF-κB signaling pathways. TRAP staining showed enhanced osteoclast activity in Lgr5 KO endplates. Western blot demonstrated reduced Collagen II and increased MMP13, p-p65 in Lgr5 KO NP cells. Immunofluorescence and immunohistochemical staining confirmed that Lgr5 deficiency increased MMP13, p-p65, and Active caspase-3, while decreasing PCNA, promoting inflammation, apoptosis, and suppressing proliferation in NP cells. Conclusions: Collectively, our results demonstrated that Lgr5 deficiency increased inflammation, disrupted ECM homeostasis and NP cell functions, which finally accelerated IDD.

Source: Frontiers in Medicine

DOI: 10.3389/fmed.2026.1939627