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Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells

Year: 2026

Type: article

Abstract: We present a protocol for isolating and culturing murine adipose progenitor cells (APCs) from adipose tissue. We describe steps for tissue dissection, unit-normalized enzymatic digestion, stromal vascular fraction (SVF) isolation, APC enrichment, and cell culture. We then detail quality control procedures that maximize cell yield, viability, and purity. This protocol combines enzymatic SVF isolation with magnetic-activated cell sorting enrichment to obtain a highly enriched APC population. It emphasizes reproducibility through unit-normalized digestion, controlled processing conditions, and standardized quality control metrics.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104901

Authors: Ghazaleh Dadashizadeh, Fadi Khalaf, Stephanie Wojtowicz-Piotrowski, Marc G. Jeschke

Institutions: McMaster University Medical Centre, Hamilton Health Sciences, McMaster University

Language: English

Topic: Mesenchymal stem cell research

Subfield: Genetics

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $1,550

OpenAlex ID: W7222477307

FWCI:

Cites: 10

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