Work

Pentraxin-3 dynamics during liver transplantation and association with early allograft dysfunction

Year: 2026

Type: article

Abstract: Introduction Liver transplantation involves significant Ischemia-Reperfusion Injury (IRI). Recipients frequently exhibit features of endothelial dysfunction, such as inflammation and coagulopathy, which may be associated with adverse outcomes, including early allograft dysfunction (EAD). Pentraxin-3 (PTX-3) is involved in vascular endothelial inflammation and dysfunction, and is synthesized by endothelial cells and leukocytes. In this retrospective cohort study, we hypothesized that PTX-3 levels are associated with EAD. Methods PTX-3 levels were quantified in plasma samples collected during liver transplantation at the following time points: start of surgery (T1); end of liver dissection (T2); after reperfusion (T3 at 15 minutes; T4 60-120 minutes). Control samples from healthy living donors were collected at the start and end of surgery. Results Plasma PTX-3 levels were higher during liver transplantation than in healthy controls at T1 (median 1.72 ng/mL, IQR 1.13-2.44 vs. 0.57 ng/mL, IQR 0.44-0.74), p <0.01, and increased over time in serial samples collected at T1, T3, and T4 ( n = 50), p <0.001. In those who developed EAD, delta-PTX-3 after reperfusion (T3 to T4) was greater (median 12.3 ng/mL, IQR 6.65-18.7, n =17) compared to those without EAD (median 6.59 ng/mL, IQR 2.87-13.2, n =34), p =0.048, but there was no significant difference in final T4 PTX-3 levels. Delta-PTX-3 after reperfusion was greater in recipients of grafts that received normothermic machine perfusion (NMP) (median 12.7 ng/mL, IQR: 7.90-16.3, n =19), vs. Living Donor Liver Transplants (median 5.79ng/mL, IQR 2.60-10.2, n =24), p =0.02. Discussion Circulating PTX-3 levels increased during liver transplantation, with the highest levels after reperfusion. This suggests IRI triggered an increase in PTX-3. After reperfusion, delta-PTX-3, but not final PTX-3, was associated with an increased incidence of EAD and was greatest in recipients of grafts after NMP, suggesting NMP may involve earlier activation of pathways downstream of IRI.

Source: Frontiers in Transplantation

DOI: 10.3389/frtra.2026.1920891

Authors: Alexandria Page, Michael P. Bokoch, Dieter Adelmann, Rachel Lin, Judith Hellman, Kerstin Kolodzie

Institutions: University of California, San Francisco, Philip R. Lee Institute for Health Policy Studies

Language: English

Topic: Organ Transplantation Techniques and Outcomes

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,353