Work

Mating cell traps to silica microbands for picoliter thin layer chromatography (pTLC) assay of phosphoinositide metabolism in single cells

Year: 2026

Type: article

Abstract: ). On average 59% ± 1% of BODIPY FL PI4P was dephosphorylated in K562 (chronic myelogenous leukemia), while significantly greater dephosphorylation (62% ± 1%) was observed in MM1S (multiple myeloma). In contrast the aggressive cell Kasumi-3 (undifferentiated acute myeloblastic leukemia) displayed significantly greater metabolism (66% ± 2%) of the PI4P reporter than other cell lines. All cell lines assessed displayed large numbers of cells with metabolic behavior far removed from that of the population average, revealing the full extent of cell-based behaviors in the cell population. Immunoassay of trapped Kasumi-3 cells followed by pTLC demonstrated the potential for pairing other assays with pTLC.

Source: Lab on a Chip

DOI: 10.1039/d6lc00694a

Authors: Ming Yao, Molly M. Prober, Yuli Wang, Katelyn Dilley, Christopher E. Sims, Nancy L. Allbritton

Institution: University of Washington

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: hybrid

Funders: National Institutes of Health, National Cancer Institute

Award: Design of an integrated microdevice to measure malignant lipid signaling in single cells from clinical samples