Work

ARCaDia-selected short DNA core positions a latent warhead for protein-gated covalent capture

Year: 2026

Type: article

Abstract: A single-round affinity/reactivity co-selection of aptamer (ARCaDia), combined with Fast String-Based Clustering (FSBC) analysis of the selection output, identified a compact 10-nucleotide DNA-warhead core motif for streptavidin-selective covalent capture, showing that protein-gated covalent chemistry can arise from a short sequence-dependent placement of a latent warhead in a biotin-sensitive reaction microenvironment. This demonstrates that functional warhead-positioning information is already present after a single selection round.

Source: RSC Chemical Biology

DOI: 10.1039/d6cb00303f

Authors: Chaohui Li, Naoyuki Miyashita, Masayasu Kuwahara, Jay Yang, Masumi Taki

Institutions: University of Electro-Communications, Kindai University, Nihon University, University of Wisconsin–Madison

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,962