Kamishoyosan reduces anxiety-like behavior by modulating GABAA receptor subunit expression in a premenstrual syndrome rat model
Year: 2026
Type: article
Abstract: Introduction: Premenstrual syndrome (PMS) and premenstrual dysphoric disorder (PMDD) involve psychological symptoms that impact women's quality of life. PMS pathogenesis is characterized by fluctuations in steroid hormones and γ-aminobutyric acid type A receptor (GABAAR) subunits. Kamishoyosan (KSS) is a Kampo medicine typically used to treat menstrual-related symptoms. This study aimed to elucidate the anxiolytic effect of KSS and its underlying mechanisms in a progesterone withdrawal (PWD)-induced PMS rat model. Methods: KSS was administered via feed mixing, and elevated plus maze test and molecular biological analysis were conducted. Results: PWD rats showed increased anxiety-like behavior and expression of GABAAR α4 and δ subunits mRNA in the hippocampal CA1 region, suggesting that decreased inhibitory GABA synaptic signaling contributes to anxiety-like behavior. KSS administration reduced anxiety-like behavior and the expression of GABAAR α4 and δ subunits, but no significant effect was observed on steroid hormone levels. Discussion: These findings suggest that KSS exerts anxiolytic effects by reversing changes in α4 and δ subunit expression without affecting steroid hormone fluctuations. The different mechanism of action of KSS compared with GABAAR agonists may provide a novel therapeutic strategy for PMS.
Source: Frontiers in Psychiatry
Institution: Tsumura (Japan)
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,801
Funder: Tsumura and Company
OpenAlex ID: W7222761787