Optimization of breeding conditions in Danionella cerebrum to advance its use as a vertebrate model
Year: 2026
Type: preprint
Abstract: We systematically evaluated tank configurations and spawning-tube parameters to improve egg production and collection efficiency in Danionella cerebrum . A shallow breeding tank with a black bottom significantly increased egg production while facilitating egg collection. Further optimization of spawning-tube parameters revealed a strong effect of tube diameter on reproductive performance, with narrower tubes improving fertilization efficiency and a 4-mm inner diameter yielding the highest egg production. Tube length also influenced breeding performance and handling efficiency. Longer tubes tended to increase egg production, whereas 5-cm tubes were more convenient for routine egg collection. The greater water volume of 10-cm tubes also enabled short-term in-tube embryo culture, potentially improving the efficiency of germline screening for transgenic founders. Notably, increasing the number of available spawning tubes did not further enhance egg production, leading us to examine patterns of spawning-tube usage. This analysis revealed a previously unrecognized preference of D. cerebrum for spawning sites previously used by other breeding pairs. Together, these findings establish practical breeding parameters that improve reproductive output and streamline egg collection and embryo handling in D. cerebrum . Summary Statement We improved breeding conditions for the tiny transparent fish Danionella cerebrum , identifying tank and tube designs that increase egg production and simplify egg collection, embryo handling, and laboratory breeding.
Language: English
Subfield: Cell Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W7222795761