RP-HPLC Method Development and Validation of Bisoprolol Fumarate and Hydrochlorothiazide in Combined Dosage Forms.
Year: 2026
Type: article
Abstract: A simple, rapid, accurate, precise, and economical RP-HPLC method was developed and validated for the simultaneous estimation of Bisoprolol Fumarate (BIS)and Hydrochlorothiazide (HCT) in pharmaceutical dosage forms. Chromatographic separation was achieved using a Shim-pack C8 column with acetonitrile: water (80:20, v/v) as the mobile phase at a flow rate of 1.0 mL/min and UV detection at 227 nm. The method showed good linearity over 2-10μg/mL for BISand 2-10μg/ml for HCT, with R² of 0.9909 and 0.9944. Accuracy was confirmed by recovery studies, with recoveries of 99.8-101.34% for BIS and 99.64-99.09% for HCT. Precision showed below 2%RSD. The method was also validated for LOD, and LOQ according to ICH guidelines and was suitable for routine analysis.
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W7222795996