Work

Addressing complex autofluorescence signatures in solid tissue samples to enhance full spectrum flow cytometry of non-immune cells

Year: 2026

Type: article

Abstract: FSFC is an emerging technology that can greatly enhance our understanding of the single-cell proteomic landscape. We demonstrate that this application is applicable to cells derived from solid tissues which have traditionally been hampered by their complex autofluorescence signatures and lack of optimized tools for non-immune cells. We present a protocol and discuss key controls that minimize the impact of unmixing errors, enabling us to resolve multiple EC subpopulations isolated from different tissues in models of chronic tissue injury. Research topic(s) vascular biology, cell heterogeneity, full spectrum flow cytometry.

Source: Biochemistry and Biophysics Reports

DOI: 10.1016/j.bbrep.2026.102845

Authors: Christina Gkantsinikoudi, Manuela Terranova-Barberio, Neil Dufton

Institutions: Queen Mary University of London, William Harvey Research Institute, Barts Cancer Institute

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,700