Bulk RNA sequencing dataset of hippocampi from RORγt transgenic mice and wild type littermates
Year: 2026
Type: data-paper
Abstract: This dataset comprises bulk RNA-sequencing data from bilateral hippocampi of six untreated, 10-week-old male mice: three heterozygous RORγt-transgenic mice and three wild-type littermates on a C57BL/6 background. Total RNA was extracted using TRIzol, and RNA integrity numbers ranged from 8.1 to 8.6. Ribosomal RNA-depleted, directional libraries were prepared from 500 ng of RNA per sample and sequenced on an Illumina NextSeq 500 with a 2 × 36-bp paired-end configuration. The received reads were 35 or 36 bases long. The 48 original compressed FASTQ files were consolidated into 12 files, preserving 83,357,563 read pairs and their pairing. Original mm10 count and RPKM matrices each contain 49,585 feature entries. An additional GRCm39 reanalysis using Ensembl release 116 provides gene-level fragment counts for 78,348 annotated features, coordinate-sorted alignments and sample-level exploratory analyses. FastQC and MultiQC reports describe raw-read quality, and principal component analysis summarizes variation across all six samples. Original sequencing data and expression matrices are deposited in NCBI Gene Expression Omnibus under accession GSE347403.
Source: Data in Brief
Institution: University of Tsukuba
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,220
OpenAlex ID: W7222809973