Primer-Template Mismatch Does Not Contribute to Irreproducibility of PCR Replicates
Year: 2026
Type: article
Abstract: Primers incorporating a range of mutations and targeting an area of the lacZ gene were tested for variability among PCR replicates. The coefficient of variance obtained from 3 PCR replicates for each mutated primer was compared with that obtained using the non-mutated primer. A control amplicon was incorporated into each reaction; variability in the ratio of the control and test amplicon integrals was used as an indicator of replicate variance. Qualitative and quantitative analysis both indicated that primer-template mismatch does not contribute significantly to replicate variability. However, very few replicates were used in this study; a larger set should be tested to confirm these findings.
Source: Open Collections
Author: Goodwin, Jonathan
Institution: The University of Western Australia
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7222825326
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