Sex specificity of root exudates and their effects on rhizosphere microorganisms in a rare and endangered plants, Taxus cuspidata
Year: 2026
Type: preprint
Abstract: Dioecious plants exhibit sexual dimorphism not only in morphological structure, physiological metabolism, and resource acquisition but also in belowground microecosystems. In this study, high-performance liquid chromatography (HPLC) and high-resolution mass spectrometry (HRMS) were employed to detect sex-specific differences in root exudates of the typical dioecious plant Taxus cuspidata between adult and juvenile stages. High-throughput sequencing was used to analyze sex- and stage-specific differences in rhizosphere microbial communities. The results showed that in the adult stage, female plants enriched 2-mercaptoethanesulfonic acid and α-keto-γ-methylthiobutyric acid, while male plants secreted more terpenoids (e.g., α-humulene) and purine metabolic intermediates (xanthine). In the juvenile stage, female plants exhibited higher expression of phosphoenolpyruvate and L-valine, whereas male plants enriched 5-methylcytosine and tricyclic sesquiterpenoid antibiotics. The diversity of rhizosphere microorganisms showed a sex-reversal pattern across different developmental stages. Correlation analysis revealed that in the adult stage, the abundance and diversity of bacteria (Proteobacteria) and fungi (Mortierellomycota) were higher in female than in male plants, which were enriched with Actinobacteria. In the juvenile stage, male plants exhibited greater diversity of both rhizosphere bacteria and fungi than female plants, with enrichment of stress-tolerant and decomposing microorganisms (e.g., Humicola ). The findings reveal different resource allocation and environmental adaptation strategies in the belowground parts of dioecious plants, which are of great value for the conservation of rare and endangered plants. Author summary Funding Source: This work was supported by National Natural Science Foundation of China (Grant No. 32272757) The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive (Genomics, Proteomics & Bioinformatics 2025) in National Genomics Data Center (Nucleic Acids Res 2025), China National Center for Bioinformation / Beijing Institute of Genomics, Chinese Academy of Sciences (GSA: CRA045178) that are publicly accessible at https://ngdc.cncb.ac.cn/gsa . data link: https://ngdc.cncb.ac.cn/gsa/browse/CRA045178
Authors: Wu Guoan, Zhang Yanwen
Institution: Eastern Liaoning University
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: green
Award: G3181199655
OpenAlex ID: W7222967412