Differential apoptosis-associated responses of neurons and glial cells to cisplatin in primary cortical cultures
Year: 2026
Type: article
Abstract: Cisplatin is a platinum-based chemotherapeutic agent widely used to treat solid tumors; however, its clinical use is limited by severe neurotoxicity.Although cisplatin shows limited blood-brain barrier permeability, accumulating evidence indicates that it induces apoptosis-associated neurotoxicity in the central nervous system.In this study, we investigated cell type-specific apoptosisassociated responses of primary cortical neurons and glial cells to cisplatin.Cytotoxicity was evaluated in EMT6 murine mammary carcinoma cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the half-maximal inhibitory concentration (IC₅₀) was determined.This concentration was then applied to primary cortical cultures derived from postnatal day 0 Balb/C mice.Apoptosis-associated responses were assessed by Western blot analysis of BCL-2 expression and immunofluorescence staining of caspase-3.Cisplatin significantly reduced BCL-2 protein levels in primary cortical cultures (p<0.01).Immunofluorescence analysis further revealed a significant increase in caspase-3-positive neurons following cisplatin exposure (p<0.01),whereas no significant change was observed in GFAP-positive glial cells (p>0.05).These findings demonstrate that cisplatin induces cell type-specific apoptosis-associated
Source: ACTA Pharmaceutica Sciencia
Authors: Neşe AyŞİt, Ayça Önal, Esra AltintaŞ, Arda KebapÇi
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7222973437