Work

Screening of Metabolite Biomarkers for Antioxidant and Glycosidase Inhibitory Activities from Oat Resources

Year: 2026

Type: article

Abstract: Oat (Avena sativa L.) is a nutritious cereal grain abundant in diverse bioactive compounds. This investigation assembled a diverse panel of 43 oat accessions, and their in vitro digestive products were evaluated for DPPH radical-scavenging capacity and α-glucosidase inhibitory activity. Metabolite profiling was conducted using UPLC-MS/MS. Tiapride, guanosine, histidine, paracetamol, and 7-aminoflunitrazepam were identified as key differential metabolites in digestates of high/low antioxidant oat accessions, while dibutyl phthalate, neocnidilide, methyltestosterone, methyl linoleate, and nafcillin were determined to be key differential metabolites in those of high/low α-glucosidase inhibition. Furthermore, a random forest model identified methyltestosterone, dibutyl phthalate, and methyl linoleate as key biomarkers for α-glucosidase inhibitory capacity. Guanosine and histidine were identified as potential biomarker candidates for antioxidant activity. This study provides a theoretical basis for the metabolic marker-assisted selection of oat accessions with improved antioxidant and anti-hyperglycemic properties.

Source: Foods

DOI: 10.3390/foods15203740

Authors: Haitao Zhou, Huiyong Yang, Yajie Zhang, Rui Hou, Yingying Zhu, Peifeng Li

Institution: Zhengzhou University of Light Industry

Language: English

Topic: Food composition and properties

Subfield: Nutrition and Dietetics

Field: Nursing

Domain: Health Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $3,499