Work

Steric attenuation of membrane-associated dimerization-induced fluorescence for cell-free biosensing

Year: 2026

Type: preprint

Abstract: We present a membrane-associated, cell-free biosensing reporter based on rapalog-induced split sfCherry reconstitution. Rapalog increased fluorescence in solution, lipid vesicles, and supported lipid bilayers. Antibodies binding to epitope tags near the dimerization interface reduced the fluorescence signal, consistent with steric interference with reporter assembly.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.64898/2026.10.19.760250

Authors: Parsa Parvizian, Hossein Moghimianavval, Allen P Liu

Institution: University of Michigan

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, University of California, Davis, National Institute of Allergy and Infectious Diseases

Award: Sensing and modulating the chemokine environment with synthetic cells