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Skin-Whitening and Anti-Photoaging Activities of Limosilactobacillus reuteri DS0333 Culture Supernatant Produced in Unripe Rubus occidentalis L. Extract-Supplemented Medium

Year: 2026

Type: article

Abstract: This study investigated the skin-whitening and anti-photoaging activities of a culture supernatant obtained by cultivating Limosilactobacillus reuteri DS0333 in chemically defined medium (CDM) supplemented with unripe Rubus occidentalis L. extract (UROE).DS0333 was cultured in UROE-supplemented medium, and the resulting DS0333/ (CDM+UROE) group was evaluated alongside all control and comparison groups.The DS0333/(CDM+UROE) culture supernatant showed enhanced DPPH and ABTS radical scavenging activities compared with all other experimental groups.In α-MSH-stimulated B16F10 cells, the culture supernatant reduced melanin content and intracellular tyrosinase activity and downregulated MITF, TRP-1, and TRP-2 expression.In UVB-irradiated human dermal fibroblasts, the culture supernatant increased type I procollagen production, reduced MMP-1 levels, and suppressed MMP-1/MMP-3 expression through attenuation of the MAPK/AP-1 signaling pathway.These results suggest that culturing DS0333 in UROEsupplemented medium enhances skin-whitening and anti-photoaging activities, potentially through cultivation-associated modification of extracellular metabolites in the culture matrix.Therefore, the DS0333/(CDM+UROE) culture supernatant may serve as a promising multifunctional cosmetic ingredient for skin applications.

Source: Journal of Microbiology and Biotechnology

DOI: 10.4014/jmb.2606.06047