Work

An improved protocol to isolate enriched immune cells from murine intestinal epithelium and lamina propria

Year: 2026

Type: article

Abstract: The intestine is home to more immune cells than any other non-lymphatic peripheral tissue, but research of these cells is stymied by the technical difficulties involved in isolating them. Here, we describe a protocol to isolate highly viable immune cells from murine intestinal epithelium and lamina propria. This protocol can be used to characterize cells by flow cytometry or single-cell transcriptomics, as in Crotts et al., or by other analysis methods.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104864

Authors: Sydney B. Crotts, Virginia Smith Shapiro

Institution: Mayo Clinic

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550