Work

Heparinization of anodized aluminum oxide (AAO) membranes for affinity separations of biocolloids

Year: 2026

Type: article

Abstract: pore area in 224 nm diameter pores. Following the heparinization of the membrane, the bioavailability of heparin is demonstrated through the capture and release of fibroblast growth factor 2 (FGF2), a protein for which heparin acts as a ligand. The capping of unreacted amines using citraconic anhydride reduces nonspecific protein binding without loss of heparin activity. The pore accessibility of colloidal particles is confirmed through the electrostatically-driven binding and release of 20 nm fluorescent amine-grafted silica nanoparticles, which are used as a model system to investigate the capture and release of biocolloids. Application of heparinized AAO membranes for the affinity capture and release of adeno associated virus 2 (AAV-2) is demonstrated in tangential flow filtration. Preliminary results for the affinity capture of AAV-2 pre-filtered culture medium capture significant AAV2 with negligible retention of host protein and DNA, paving a path for further optimization of viral vector purification.

Source: RSC Advances

DOI: 10.1039/d6ra07687d