Work

Tools for in vivo labelling of ALFA-tagged proteins in C. elegans.

Year: 2026

Type: article

Abstract: Understanding protein function requires tools to visualize and manipulate proteins in vivo.NanoTags, short epitope tags that are recognized by single-chain antibodies, are an emerging method for versatile in vivo labelling and manipulation of endogenous proteins.The short (13 amino acid) ALFA tag has been shown to allow labelling of endogenous proteins in several model systems, including C. elegans.Here we describe the generation and validation of transgenes to facilitate global and tissue-specific visualization of ALFA-tagged endogenous proteins in C. elegans.We propose a modular NanoTag system as a powerful approach for investigating protein functions in vivo in C. elegans.

Source: microPublication biology

DOI: 10.17912/micropub.biology.002419

Authors: Irini Topalidou, Nicolas Lehrbach

Institution: Fred Hutch Cancer Center

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: National Institutes of Health, National Institute of General Medical Sciences, Office of Research Infrastructure Programs, National Institutes of Health

Awards: G1994913649, G3770255479, Caenorhabditis Genetics Center, Regulation of Proteasome Capacity