Response Surface Optimization of Ultrasound‐Assisted Deep Eutectic Solvent Extraction of Bioactive Polysaccharides From Sword Bean ( Canavalia gladiata (Jacq.) DC.) Pods
Year: 2026
Type: article
Abstract: ABSTRACT Safe and functional natural active ingredients are a research hotspot in early intervention studies of chronic diseases. This study focuses on a polysaccharide (CGPs‑1) from sword bean ( Canavalia gladiata (Jacq.) DC.) pods. Its optimal ultrasound‐assisted extraction conditions were achieved with choline chloride/oxalic acid DES (2:1), 34% water, 21 mL/g, 65°C, 29 min at 280 W; the actual yield (33.24%) was consistent with the predicted value (33.96%). CGPs‑1 was identified as a pyran‑type polysaccharide, comprising rhamnose (Rha), glucuronic acid (GlcA), and galacturonic acid (GalA) in a molar ratio of 1.08:1:1.78. CGPs‑1 had IC 50 values of 0.157 mg/mL (α‑glucosidase), 0.083 mg/mL (α‑amylase), 31.18 mg/mL (DPPH radicals), and 2.264 mg/mL (ABTS radicals), inhibited LPS‑triggered inflammatory response and LPS‑mediated neurotoxicity in BV2 cells, and also suppressed MPTP‐induced inflammatory response in zebrafish larvae. Results indicate that CGPs‑1 exhibits antioxidant, hypoglycemic, anti‑neuroinflammatory and neuroprotective potentials, providing preliminary experimental support for further research and the development of functional‑food‑related products derived from sword bean pods.
Source: Chemistry & Biodiversity
DOI: 10.1002/cbdv.71766
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,880
OpenAlex ID: W7223096389