Work

Protocol for constructing barcoded Saccharomyces cerevisiae strains and performing pooled mating experiments

Year: 2026

Type: article

Abstract: Barcoded Saccharomyces cerevisiae strains enable pooled genetic analyses across many genotypes. Here, we describe a protocol for generating stable barcoded haploid strains from natural diploid isolates. The workflow includes high-throughput transformation, selection and validation of transformants, followed by sporulation and recovery of stable haploids. We also describe phenotypic and molecular validation methods. An optional en masse mating procedure generates pooled diploid offspring. For complete details on the use and execution of this protocol, please refer to Strauss et al. 1 1. Strauss, S.K. ∙ Golomb, R. ∙ Khoury, D. ... Quantitative genetics of natural S. cerevisiae strains upon mating reveal heritable determinants of fitness and differential mating affinities Cell Rep. 2026; 45 , 116972 Full Text Full Text (PDF) Scopus (1) PubMed Google Scholar

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104905

Authors: Sivan Kaminski Strauss, Yitzhak Pilpel, Orna Dahan

Institution: Weizmann Institute of Science

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funders: Minerva Foundation, University of Toronto