Year: 2026
Type: preprint
Abstract: Routine inspection of raw mass spectrometry data is limited to installed desktop software which often needs high memory, security updates, compatible operating system, and hard-drive space. Existing web-based tools require uploading raw data files to a server. To address this, we introduce mzView, a zero-installation web application featuring client-side modules for visualizing mass spectrometry data files covering Data Dependent Acquisition (DDA), Data Independent Acquisition (DIA), Imaging, and Ion-mobility acquisitions. mzView supports mzML, imzML, MSP, Bruker (.d) file formats. It enables multi-target extraction ion chromatograms (EICs) with MS2-event overlays and isotope- and adduct-driven EIC visualization ; global filtering of DDA MS2 spectra by precursor and fragment ions; DIA fragment EICs by isolation window and fragment co-occurrence; and rapid in-browser MSP library parsing and filtering. Ion-mobility and imaging data are visualized using WebGL GPU based faster rendering. To assist in spectra annotation, mzView searches spectra against the IDSL spectral database of over 50,000 compounds curated from publicly available mass spectral databases, scored by spectral entropy and cosine similarity with interactive mirror plots. Accepted hits can be saved to a browser-local annotation library, edited, re-searched, and exported as MSP files. Demonstrated on publicly available DDA and DIA data files of NIST SRM 1950 plasma acquired on a SCIEX ZenoTOF 8600, and additional data collected using Bruker timsTOF Metabo and MALDI- Pro Ultra 2 instruments, mzView readily handles files up to 35 GB, with indexing, filtering, and EIC extraction completing in less than 60 seconds. mzView is freely available at https://mzview.org (License: CC-BY).
Source: ChemRxiv
Institution: Icahn School of Medicine at Mount Sinai
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7223170804