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Low-affinity exchangeable probes for super-resolution and long-term fluorescence imaging

Year: 2026
Type: review
Abstract: , antibodies, nanobodies, or affimers). Emerging approaches also employ peptides, proteins, nucleic acid aptamers, and small molecules as low-affinity ligands. Low-affinity labelling already enables imaging with resolution down to ∼1 nm, but it remains rather slow and mainly compatible with fixed cells. We anticipate that this field will continue to expand rapidly, driven on the one hand by new concepts in fluorogenic dye design and reversible labelling strategies and, on the other hand, by the growing range of applications in advanced fluorescence microscopy.
DOI: 10.1039/d6sc05417j
Language: English
Subfield: Biophysics
Open Access status: diamond
Funders: European Commission, Agence Nationale de la Recherche, Université de Strasbourg, China Scholarship Council, Centre National de la Recherche Scientifique, HORIZON EUROPE Framework Programme, H2020 European Research Council
Awards: G1800105685, Par-delà les frontières, l'Université de Strasbourg, G2255650664, G2694206344, G6364173891, Complex Systems Chemistry International Graduate School, Amplified biosensors for metabolites based on fluorescent nanoantennas
OpenAlex ID: W7223171846
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