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Nanoparticles improve targeting and efficacy of endocytosis inhibitors

Year: 2026
Type: article
Abstract: a linear Knoevenagel condensation pathway and structurally confirmed by NMR and LC-MS spectroscopy. Molecular docking predicted more favorable interactions of Les-6623 and Les-6632 with dynamin-2 than those predicted for chlorpromazine, a classical endocytosis inhibitor. PEGylated gold nanoparticles were functionalized with the inhibitors, yielding formulations characterized by UV-Vis, fluorescence spectroscopy, ATR-FTIR, DLS, and TEM analyses. Functional assays in SH-SY5Y and BEAS-2B cells showed that AuNP_Les-6623 and AuNP_Les-6632 reduced the cellular fluorescence associated with transferrin, HIV-1 Tat, and SARS-CoV-2 Spike protein under the experimental conditions tested. Free chlorpromazine was included as a reference inhibitor. These findings support the biological activity of the investigated nanoparticle formulations under the tested conditions, while further format-matched controls are required to determine the specific contribution of nanoparticle presentation to the observed activity.
Source: RSC Advances
DOI: 10.1039/d6ra07148a
Authors: Olga Klaudia Szewczyk-Roszczenko, Piotr Roszczenko, Iwona Misztalewska-Turkowicz, Rostyslav Dudchak, Ihor Yushyn, Yulia Shepeta, Serhii Holota, Anna Bielawska, Robert Czarnomysy, Roman Lesyk, Agnieszka Wilczewska, Yegor Vassetzky, Krzysztof Bielawski
Institutions: Medical University of Białystok, University of Białystok, Danylo Halytsky Lviv National Medical University, National Pirogov Memorial Medical University, Vinnytsya, University of Information Technology and Management in Rzeszow, Centre National de la Recherche Scientifique, Université Paris-Saclay, Institut Gustave Roussy, Aspects métaboliques et systémiques de l'oncogénèse pour de nouvelles approches thérapeutiques
Language: English
Subfield: Biomaterials
Open Access status: gold
Estimated APC paid: $1,042
OpenAlex ID: W7223173628
FWCI:
Cites: 30
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Related to: 10