Work

Low-latency axial depth estimation of 2.82 ms using light field microscopy

Year: 2026

Type: article

Abstract: Low-latency axial depth estimation is important for automated microscopic observation and feedback control because estimation delay degrades feedback-control performance and prolongs defocus, thereby reducing the quality of microscopic observation. This paper presents a low-latency axial position estimation method based on light field microscopy for rapid optimal focal plane determination. A CPU–GPU implementation achieved a measured end-to-end estimation latency of 2.82 ± 0.58 ms across 100 trials. An additional experiment quantitatively validated x-direction displacement estimation using a fluorescent microsphere aggregate, and high-speed closed-loop autofocus was demonstrated using a USAF target and fluorescent microspheres.

Source: Optics Express

DOI: 10.1364/oe.613439

Authors: Jiaqi Li, Yu Cao, Hiromasa Oku

Institution: Gunma University

Language: English

Topic: Image Processing Techniques and Applications

Subfield: Media Technology

Field: Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funders: Gunma University, Japan Science and Technology Agency