Work

Validation of micrometry as a low-cost method for chorion thickness measurement in Atlantic salmon eggs (Salmo salar)

Year: 2026

Type: article

Abstract: Accurate measurement of chorion thickness is essential for evaluating egg quality and susceptibility to saprolegniasis in salmonids. Chorion thickness is commonly assessed using techniques such as scanning electron microscopy (SEM) and optical microscopy (OM) following histological processing. However, ultrastructural techniques such as electron and optical microscopy are costly, time-consuming, and require specialized equipment and highly trained personnel. The present study aimed to evaluate the validity of micrometry as a rapid and low-cost alternative for chorion thickness assessment in Atlantic salmon eggs. Micrometry showed values comparable to optical microscopy and acceptable variability relative to biologically relevant differences associated with egg susceptibility. These results support the use of digital micrometry for routine egg quality assessment, as the method requires minimal equipment, can be performed rapidly, and does not depend on specialized microscopy expertise.•Validates digital micrometry as a rapid and low-cost method for measuring chorion thickness in Atlantic salmon eggs.•Demonstrates agreement between micrometry and optical microscopy for routine egg quality assessment.•Provides an accessible alternative that can be implemented without advanced technical expertise and at low cost.

Source: MethodsX

DOI: 10.1016/j.mex.2026.104226

Authors: Christian Ellwanger-Reyes, Sebastián Ávila, Evelyn Provoste Forcael, Wellison Amorim Pereira, Elías Figueroa Villalobos, Iván Valdebenito

Institutions: Universidad Católica de Temuco, Universidade de São Paulo

Language: English

Topic: Aquaculture disease management and microbiota

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $1,260