MR Imaging-based Evaluation of Cerebrospinal Fluid Dynamics in Cynomolgus Monkeys Using 17O-labeled Saline and Gd-DOTA
Year: 2026
Type: article
Abstract: PURPOSE: O-labeled saline and gadolinium (Gd)-based contrast agents have been applied to investigate CSF dynamics using MRI in preclinical settings, studies in non-human primates remain limited. This study evaluated the feasibility of visualizing CSF kinetics in cynomolgus monkeys using these 2 tracers. METHODS: O-labeled saline protocol, T2-weighted images were acquired every minute for 20 min. For the Gd-DOTA protocol, T1-weighted images were acquired at 10 min, 30 min, 1 hr, 4 hr, and 24 hr after administration. Signal intensities (SIs) within CSF and parenchymal regions were analyzed. RESULTS: O-labeled saline injection, the % change in SI in the cisterna magna peaked rapidly at 2-3 min and subsequently propagated caudally along the cervical spinal canal without detectable parenchymal uptake. In contrast, after Gd-DOTA administration, the % change in SI in the cisterna magna reached its maximum at 1-4 hr, with widespread distribution into ventricles, cisterns, and spinal canal. Gd-DOTA clearance via the cribriform plate was evident. Parenchymal enhancement was observed in the cerebellum, pons, and cingulate gyrus with greater signal change in superficial compared to sub-superficial layers. Marked enhancement was also observed in gray matter surrounding the spinal central canal at 4 hr. CONCLUSION: O-labeled saline enabled rapid visualization of bulk CSF flow, Gd-DOTA provided insights into slower CSF distribution and parenchymal transport. These findings provide a translational platform for future investigations into age- and disease-related alterations in CSF dynamics.
Institution: Shiga University of Medical Science
Language: English
Subfield: Clinical Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7223198392